NCI-H1703-Luc
CBP30028L
| I. Introduction | |
| Host Cell: | NCI-H1703 |
| Expressed gene: | Luciferase |
| Stability: | 32 passages(in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640+10%FBS+2μg/ml Puromycin |
| Mycoplasma Status: | Negative |
| Storage: | Liquid nitrogen |
| II. Description of Host Cell Line | |
| Organism: | Human |
| Tissue: | Lung |
| Disease: | Colorectal adenocarcinoma |
| Morphology: | Epithelial |
| Growth Properties: | Adherent |
| III. Representative Data | |
|
Figure 1. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001(we strongly recommend purchasing from Cobioer).The number of Luminescence signal is 585±22(Mean±SD) on NCI-H1703 cells while 8211069±204759(Mean±SD) on NCI-H1703-Luc cells. |
|
| IV. Stablity Test | |
|
Figure 2. Stability test results. NCI-H1703-Luc cells showed at least 32 passage stability with similar luminescence unit. |
|
| V. In VIVO Data | |
|
Index |
Content |
|
Animal |
NOD SCID, 7-8weeks, Female |
|
Implanted Position |
Intracranial injection |
|
Injected Cell Numbers |
1×10^5/4 ul |
|
Matrix gel |
Without matrix gel |
1. Weight Change
Figure 3. NCI-H1703-luc Mean Body Weight ± SEM.
2. Tumor Flux
Figure 4. Total flux NCI-H1703-luc.
NOTE:The above data was provided by Cobioer (cell data) and Truway Bio (in vivo data model).