4ig-B7H3(CD276)/CT26
CBPG0018
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| I. Background | |
| 免疫调节蛋白的 B7 家族是结构相关的细胞表面蛋白，在抗原呈递细胞/肿瘤细胞内和 肿瘤/免疫细胞之间的信号传导中起着核心作用。B7 家族成员（包括 CTLA-4、PD-1 和 PD-L1）之间的相互作用介导的免疫检查点机制可以产生致瘤和共调节功能。B7-H3 蛋白 表达与许多实体瘤（包括宫颈癌、卵巢癌和乳腺癌等）的不良预后具有相关性。B7-H3 （CD276）有两种异构体（2Ig 和 4Ig），没有确定的同源受体，B7-H3 在多种恶性肿瘤中 均有表达，与恶性肿瘤的生长、转移、复发、预后不良等因素密切相关。B7-H3 可下调Ｔ 辅助 1 型介导的免疫反应抑制 CD4+T 细胞活化，并抑制细胞因子的产生，发挥促进癌细胞 免疫逃逸的作用。 | |
| II. Introduction | |
| Host Cell: | CT26 |
| Expressed gene: | h4ig-B7H3(CD276) |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640+10%FBS+10μg/ml Puromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| III. Description of Host Cell Line | |
| Organism: | Mouse |
| Tissue: | colon, metastatic |
| Morphology: | N/A |
| Growth Properties: | monolayer |
IV. Representative Data Figure 1. Recombinant h4ig-B7H3(CD276) stably expressing 4ig-B7H3(CD276). |
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| V. In VIVO Data | |
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Index |
Content |
|
Animal |
BALB/C, 6weeks, female |
|
Implanted Position |
Subcutaneous (right flank region ) |
|
Injected Cell Numbers |
5×10^5/0.1ml |
|
Matrix gel |
Without matrix gel |
1. Weight Change
Figure 2. 4ig-B7H3(CD276)/CT26 Mean Body Weight ± SEM.
2. Tumor Size
Figure 3.4ig-B7H3(CD276)/CT26 Mean Tumor Volume ± SEM .
NOTE：The above data was provided by Cobioer (cell data) and Truway Bio (in vivo data model).
