hMET/LL/2
CBPG0146
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| I. Background | |||
| C-Met 属于受体酪氨酸激酶家族，是一种异二聚体，由高度糖基化的细胞外 50 kDa α 链和跨膜 140 kDa β链组成，顺利获得二硫键连接在一起。作为一种天然的 HGF 受体，它表达 于各种上皮细胞的表面，并促进肿瘤的发生。与 HGF 结合后，两个亚基二聚化，导致两个 酪氨酸残基（Tyr1234 和 Tyr1235 ）自磷酸化。酪氨酸残基的自磷酸化导致一个激活的多功 能停靠位点，该位点由 SH2 结构域、PTB 结构域和 Met 结合结构域 （MDB） 组成，该结 构域募集接头分子。 | |||
| II. Introduction | |||
| Host Cell: | LL/2 | ||
| Expressed gene: | hMET | ||
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) | ||
| Freeze Medium: | 90% FBS+10% DMSO | ||
| Culture Medium: | DMEM+10%FBS+10 μg/ml blasticidin | ||
| Mycoplasma Testing: | Negative | ||
| Storage: | Liquid nitrogen | ||
| III. Description of Host Cell Line | |||
| Organism: | Mus musculus, mouse | ||
| Tissue: | lung | ||
| Morphology: | rounded - loosely attached or floating | ||
| Growth Properties: | mixed, adherent and suspension | ||
| IV. Representative Data | |||
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Figure 1. Recombinant hMET/LL/2 stably expressing hMET. |
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