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    kdpay钱包(中国) /药靶模型 /示踪细胞 / /NCI-H1975-Luc

    NCI-H1975-Luc

    CBP30029L

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    I. Introduction
    Host Cell: NCI-H1975 EGFR p.L858R T790M
    Expressed gene: Luciferase
    Stability: 32 passages(in-house test, that not means the cell line will be instable beyond the passages we tested.)
    Freeze Medium: 90% FBS+10% DMSO
    Culture Medium: RPMI-1640+10%FBS+2μg/ml Puromycin
    Mycoplasma Status: Negative
    Storage: Liquid nitrogen
     
    II. Description of Host Cell Line
    Organism: Homo sapiens,human
    Tissue: Lung
    Disease: Adenocarcinoma;non-small cell lung cancer
    Morphology: Epithelial
    Growth Properties: Adherent
     
    III. Representative Data
    CBP30029L 信号

    Figure 1. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System..
    The number of Luminescence signal is 549±33(Mean±SD) on NCI-H1975 cells while 641009±23361(Mean±SD) on NCI-H1975-Luc cells.

     
    IV. Stablity Test
    CBP30029L 稳定性

    Figure 2. Stability test results. NCI-H1975-Luc cells showed at least 32 passage stability with similar luminescence unit.

     
    V. In VIVO Data

    Index

    Content

    Animal

    BALB/c Nude, 11-12weeks, Female

    Implanted Position

    Intracranial injection

    Injected Cell Numbers

    1×10^5/4ul

    Matrix gel

    Without matrix gel


    1. Weight Change 



    Figure 3.
    NCI-H1975-Luc Mean Body Weight ± SEM.

     

    2. Total Flux

     



    Figure 4. Total flux NCI-H1975-Luc.


    NOTE:The above data was provided by Cobioer (cell data) and Truway Bio (in vivo data model).


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